Which practice helps minimize hemolysis in a blood specimen?

• A. Use vigorous mixing
• B. Draw from a small vein
• C. Use proper venipuncture technique with appropriate needle gauge and gentle handling
• D. Expose to cold temperatures

Answer: (C)

Hemolysis occurs when red blood cells are damaged during collection and handling, so the best protection is to minimize mechanical trauma. Using the proper venipuncture technique with an appropriate needle gauge reduces shear forces as blood enters the collection tube, and gentle handling throughout collection and transport prevents cell rupture. This combination preserves cell integrity and yields more reliable test results. Practices that can promote hemolysis—such as vigorous mixing, drawing from a very small vein with more manipulation, or exposing the specimen to cold temperatures—are less protective and should be avoided.