Where is the counting region located in a feathered edge smear?

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Multiple Choice

Where is the counting region located in a feathered edge smear?

Explanation:
Counting region is placed in the feathered edge because that part of the smear is the thinnest and most evenly distributed, making individual cells or colonies easier to distinguish. In the center of the smear, cells tend to be crowded and overlapped, which leads to inaccurate counts. The outer edge beyond the smear has little sample to count, so it isn’t suitable either. The feathered edge provides a balance where the sample density is low enough for reliable enumeration while still being part of the smear.

Counting region is placed in the feathered edge because that part of the smear is the thinnest and most evenly distributed, making individual cells or colonies easier to distinguish. In the center of the smear, cells tend to be crowded and overlapped, which leads to inaccurate counts. The outer edge beyond the smear has little sample to count, so it isn’t suitable either. The feathered edge provides a balance where the sample density is low enough for reliable enumeration while still being part of the smear.

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